Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands.
نویسندگان
چکیده
Single-stranded adenovirus-associated virus type 2 deoxyribonucleic acid (AAV-2 DNA) has been isolated from the virion after enzymatic pretreatment of the particles by heating at 53 C for 1 hr in 0.015 m NaCl plus 0.0015 m sodium citrate in the presence of 1% sodium dodecyl sulfate. Double-stranded AAV-2 DNA present as a marker is not denatured by this treatment. AAV-2 single-stranded DNA is composed of two complementary species which can be separated in neutral CsCl when 5-bromodeoxyuridine has been substituted for thymidine in the DNA. The present report is the first documented instance of the separation of complementary strands of an animal virus DNA.
منابع مشابه
Self-complementarity of terminal sequences within plus or minus strands of adenovirus-associated virus DNA.
At least 70% of plus or minus strands of adenovirus-associated virus DNA contain self-complementary sequences at or near their termini. Self-annealing of these sequences generates circular molecules that are closed by duplex, hydrogen-bonded segments. The self-annealed segments are sensitive to exonuclease III and have a thermal stability comparable to that of double-stranded DNA molecules. Len...
متن کاملSimian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule.
The E46(+) strain of Adenovirus 7 is a mixed-virus population containing defective Adenoviurs 7-SV40 hybrid particles and helper, nonhybrid Adenovirus 7 particles. We have applied electron microscopic mapping techniques to obtain a physical map of the genome of the hybrid particles present in E46(+)PL1, a substrain of E46(+) derived from a single two-hit plaque. DNA molecules extracted from pur...
متن کاملReplication of double-stranded RNA in particles of Penicillium stoloniferum virus S.
RNA polymerase activity was assayed in different particle classes of Penicillium stoloniferum virus S. RNA polymerase activity was found to be associated with H particles, which contain double-stranded RNA and single-stranded RNA, but not with L particles, which contain only double-stranded RNA and not with M particles, which contain only single-stranded RNA. In H particles the reaction occurre...
متن کاملElectrophoretic strand separation of long DNAs with poly (U,G) in agarose gels.
We have found that binding of poly(U,G) to single-stranded DNA decreases its mobility in 0.3% agarose gels. Differential binding to the complementary strands of denatured duplex DNA provides a simple method for strand separation. The method is shown to work with bacteriophage lambda DNA, adenovirus DNA and mtDNA for Tetrahymena pyriformis. In all cases the strand that binds more poly(U,G) in Cs...
متن کاملDNA forms of the geminivirus African cassava mosaic virus consistent with a rolling circle mechanism of replication.
We have analysed DNA from African cassava mosaic virus (ACMV)-infected Nicotiana benthamiana by two-dimensional agarose gel electrophoresis and detected ACMV-specific DNAs by blot-hybridisation. ACMV DNA forms including the previously characterised single-stranded, open-circular, linear and supercoiled DNAs along with five previously uncharacterised heterogeneous DNAs (H1-H5) were resolved. The...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of virology
دوره 5 6 شماره
صفحات -
تاریخ انتشار 1970